Dio easily takes the crown. This boy hate is everlasting. Even as a child he was a hater to JoJo and his entire bloodline before grabbing the stone mask and turning into pure evil.
"So this is the brat I must usurp. He wears his heart on his sleeve and is quick to anger....This pampered fool is nothing for the likes of Dio Brando!"
"I'll defeat this boy in his own home. He'll understand that his place in this world is under my heel."
William Zepelli: "Tell me to undo your injuries, how many innocent lives did you consume?"
Dio: "I don't know. How many loaves of bread have you eaten in your lifetime?"
"Human ability is extremely limited and finite. Your Hamon training avails you nothing. It's USELESS, USELESS, USELESS, UUUSSSEELLLESSS!! A MERE MONKEY COULD NEVER DEFEAT A LION!!!"
"You thought your first would be Jojo but it was I DIO!!!
^This is the coldest one IMO. If you know you know